Multiplex PCR

One test, several results

Molecular diagnostics is now easier. Our exclusive DNA amplification technology, QuickTAAG®, allows the simultaneous amplification of multiple targets in a single reaction, making the diagnostic even more accurate. Assays using QuickTAAG® technology are highly sensitive, specific and reproducible.

The QuickTAAG® products are based proprietary multiplex PCR technology, which allows the simultaneous detection and identification of multiple targets in one reaction. QuickTAAG® kits eliminate artifacts easily found in conventional PCR, no more struggle with primer design, PCR optimization and non-specific PCR products. Furthermore, QuickTAAG® kits include an internal positive control, which prevents misjudgements arising from erroneous testing, hence you obtain the most confident results.

Clear PCR

PCR carryover contamination, not a problem anymore

All our DNA amplification kits incorporate a unique “contamination-proof“ technology, ClearPCR, which can be activated in case of contamination of the reagents or used routinely to avoid potential contamina¬tions.

The current methods for preventing PCR amplification carryover contamination, such as, the enzymatic inactivation with uracil-N-glycosylase (UNG), has several disadvantages: uracil is not a natural building block for DNA, making PCR amplification less efficient when it is used¹ and using dUTP is shown to be inefficient on GC rich templates¹. Additionally under certain circumstances, UNG may not be completely inactivated and the residual enzymatic activity may be enough to degrade amplification products generated during the early amplification cycles².

Unlike the current methods for preventing PCR amplification carryover contamination the key of the ClearPCR technology is in the primers. All QuickTAAG® primers plus the ClearPCR solution significantly reduce re-amplification of PCR products (Fig. 1).

1. Jaber Aslanzadeh. Preventing PCR Amplification Carryover Contamination in a Clinical Laboratory. Annals of Clinical & Laboratory Science 2004;34:389-396.
2. Ritzler M, Perschil L, Altwegg M. Influence of residual uracil-DNA glycosylase activity on the electrophoretic migration of dUTP-containing PCR products. J Microbiol Meth 1999;35:73–76.

DNA SeqFinder

Assays more specific impossible, simultaneous identification of multiple targets through DNA sequencing

The DNA SeqFinder Software, through the use of advanced algorithms (patent pending), allows analyzing mixed and complex DNA chromatogram obtained by multiplex sequencing of several targets simultaneously, thus allowing the simultaneous detection and identification of the pathogens contained in a biological sample.

UniTAAG® Project

The most simple and accurate taxonomic assignment

Accurate taxonomic assignment plays a critical role in academic, clinical and industrial areas. TAAG Diagnostics is developing the pioneering UniTAAG® technology, the first universal and comprehensive methodology to infer phylogenetic relationships between organisms. The UniTAAG® technology will be a fast and easy-to-use taxonomic assignment system suitable for either small or large scale projects.

The UniTAAG® workflow is quite simple: DNA extraction from isolated microorganism, one multiplex PCR reaction for the simultaneous amplification of multiple loci with differential evolutionary rates, sequencing and analyzing, using the DNA SeqFinder Software, the mixed DNA chromatogram in order to accurately identify the organism.